Testing an Antibiotic Using a Disk Diffusion Assay - Kirby Bauer Method

At GoldBio, we make sure each of our products are thoroughly tested to the highest standards. In addition to a typical certificate of analysis (COA), we also test our antibiotics through a modified version of the Kirby-Bauer method, also known as the disk diffusion method. The test compares the antibiotic's ability to prevent bacterial growth to a common set of standards created by the Clinical Laboratory Standards Institute (CLSI) in order to assure its functionality at a certain concentration. As a control, we use a strain sensitive to the antibiotic to determine its effectiveness and a resistant strain to assure it is the correct substance. Each lot is tested with this procedure. Visit our website at: https://www.goldbio.com **Update November 2014** Rather than going through the steps addressed in the video, this blog will just highlight important things to consider so you’re prepared to test when the time comes - https://www.goldbio.com/articles/article/old-antibiotics-wont-spoil-research-with-this-test **Update August 2017** Here is the equipment used in this video: -6 mm filter paper discs -sterile petri dishes -agar plates with no antibiotic -sensitive and resistant bacterial cultures -antibiotic to test and antibiotic for control Other items out on the bench is a Bunsen burner, lighter, tube racks, thermometer, pipette, pipette tips, antibiotics solution, forceps, ethanol, flow hood (optional) and a marker to label discs View GoldBio high-quality research antibiotics here: https://www.goldbio.com/collection/antibiotics-and-selection Popular research antibiotics for selection in laboratories: Ampicillin: https://www.goldbio.com/product/887/ampicillin-sodium-usp-grade Carbenicillin: https://www.goldbio.com/product/960/carbenicillin-disodium Chloramphenicol: https://www.goldbio.com/product/981/chloramphenicol-usp-grade G-418: https://www.goldbio.com/product/3022/g418-sulfate Hygromycin: https://www.goldbio.com/product/2338/hygromycin-b Kanamycin: https://www.goldbio.com/product/1393/kanamycin-monosulfate-usp-grade Neomycin: https://www.goldbio.com/product/3292/neomycin-sulfate-usp-grade Nourseothricin (NTC, cloNAT): https://www.goldbio.com/product/1678/nourseothricin-sulfate-streptothricin-sulfate Phosphinothricin:https://www.goldbio.com/product/1728/phosphinothricin Puromycin:https://www.goldbio.com/product/2691/puromycin-dihydrochloride Rifampicin: https://www.goldbio.com/product/1837/rifampicin Spectinomycin: https://www.goldbio.com/product/1882/spectinomycin-dihydrochloride-pentahydrate-usp-grade

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